Direct observation of the microfiltration of yeast cells at the micro-scale: Characterization of cake properties

Alberto Valencia (1) , Claude Lemen (2) , Christophe Ellero (2) , Christine Lafforgue-Baldas (2) , Jeffrey F. Morris (3) , Philippe Schmitz (2)

1 FERMAT - Fédération de Recherche Fluides, Energie, Réacteurs, Matériaux et Transferts
2 TBI - Toulouse Biotechnology Institute
3 CCNY - City College of New York [CUNY]

This study examines the accumulation of yeast cells at the membrane surface and the morphology of the formed cake through microscale monitoring and analysis of the microfiltration process. An original dead-end microfiltration device with a model membrane was designed and coupled with an optical imaging system to provide direct observation from the side, allowing in-situ real time study of the filtration operation (Valencia et al. 2020 [1]). Here, the deposition of yeast cells, monodispersed and polydispersed particles, in the same size range that yeast cells, was analyzed. Image processing was used to perform a quantitative characterization of cake morphological properties in terms of height, porosity, permeability, Kozeny coefficient and specific resistance. The cakes formed by monodispersed spherical and non-spherical rigid particles exhibit a similar incompressible behavior with higher porosity than yeast cakes, with mean porosity values of 0.38 for the rigid particles and 0.15 for the yeast at the end of the filtration run, respectively. The cake obtained by the microfiltration of a model suspension of polydispersed particles close to yeast size and shape is more compact (porosity of 0.29) and less permeable. However, polydispersity does not fully explain yeast cake properties, in particular its compressibility. Indeed, the yeast cake has a high compressibility index n = 1.1, which is reflected in a significant volume expansion of the yeast cake after transmembrane pressure was removed.

Mots clés: Microfiltration; Yeast; Model particle; Compressible cake

Domaines: Biotechnologies; Génie chimique

Alberto Valencia, Claude Lemen, Christophe Ellero, Christine Lafforgue-Baldas, Jeffrey F. Morris, et al.. Direct observation of the microfiltration of yeast cells at the micro-scale: Characterization of cake properties. Separation and Purification Technology, 2022, 298, pp.121614. ⟨10.1016/j.seppur.2022.121614⟩. ⟨hal-03796824⟩